Fig 1: Xenograft tumour suppression by the AID2 system.a mAID-BRD4 or TOP2-mAC HCT116 cells expressing OsTIR1(F74G) were treated with 1 µM 5-Ph-IAA and sampled at the indicated time points. Proteins were separated and detected using a specific antibody. Tubulin was used as a loading control. We repeated this experiment twice and obtained similar results. b Experimental time-course diagram showing the xenograft assay. c Graph showing mAID-BRD4 xenograft tumour growth. Tumour volume was checked on the indicated days. Data are presented as mean values ± SD (n = 5 animals). d Graph showing the tumour weight on day 14. mAID-BRD4 xenograft tumour was taken and weighed. Data are presented as mean values ± SD (n = 5 animals, two-tailed t-test). e Graph showing TOP2A-mAC xenograft tumour growth. Tumour volume was checked on the indicated days. Data are presented as mean values ± SD (n = 4 animals). f Graph showing the tumour weight on day 14. TOP2A-mAC xenograft tumour was taken and weighed. Data are presented as mean values ± SD (n = 4 animals, two-tailed t-test).